2003
Volume
3, Number 3/4, pp. 85–88
Z. Kuchukashvili,† G.
Davitaia,†
I. Gorozia,† G. Kvesitadze,‡ E. Lomidze‡ and G.
Khatisashvili‡
†Iv. Javakhishvili Tbilisi State University, 2 University St, 0128
Tbilisi, Georgia
‡Durmishidze Institute of Biochemistry and
Biotechnology, Georgian Academy of Science,s D. Agmasheneblis Kheivani 10 km,
0159 Tbilisi, Georgia
Antioxidant
characteristics of phenolic compounds of Satureia hortensis
The antioxidant characteristics of the phenolic water fraction of the
annual plant Satureia hortensis have been studied. Rabbit liver
microsomes served as the oxidative system and arachidonic acid as a substrate.
By administering carbon monoxide, NADPH and dimethylaniline the participation of
cytochrome P450-dependent monoöxygenase in
the oxidation process was examined. Aspirin and phenolic compounds of Satureia
hortensis were used to study other oxidative systems of rabbit liver
microsomes characterized by arachidonic acid consumption. The results obtained
showed that arachidonic acid was oxidized in microsomes and the process was
intensified in the presence of NADPH. Cytochrome P450-dependent monoöxygenase is not the only oxidizer of arachidonic
acid: other oxidative systems of rabbit liver microsomes also take part in this
process. The phenolic fraction of Satureia hortensis inhibits more
strongly than carbon monoxide, a standard inhibitor of cytochrome
P450-dependent monoöxygenase and other
microsomal oxidative systems. The inhibitory effect manifested in vitro
correlates with the analogous action of aspirin—one of the primary
representatives of nonsteroid anti-inflammatory drugs.
Keywords: arachidonic acid, cytochrome P450 monoöxygenase, inhibition, microsomes, phenolic compounds, Satureia hortensis