The Journal of Biological Physics and Chemistry

2009

Volume 9, Number 2, p.p. 83–87


Biophysical approach to evaluate DNA transfer by PLGA nanoparticles

T. Mdzinarashvili,1, 2 M. Khvedelidze,1, 2 T. Partskhaladze,1 N. Nafee,3 U.F. Schaefer,3 C.-M. Lehr,3 and M. Schneider4

1 Department of Physics, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, 3 Chavchavadze Ave, 0128 Tbilisi, Georgia
2 Institute of Molecular Biology and Biophysics, 12 Gotua St, 0160 Tbilisi, Georgia
3 Departments of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Postfach 151150, D-66041 Saarbrücken, Germany
4 Department of Pharmaceutical Nanotechnology, Saarland University, Postfach 151150, D-66041 Saarbrücken, Germany

A survey of nanoparticles used for drug delivery is provided, focusing on poly(lactic-co-glycolic acid) (PLGA). The interaction between DNA and PLGA nanoparticles has been investigated. A complex structure comprising the nanoparticles and DNA chains has been unambiguously demonstrated, using a new approach involving spectrophotometric and ultracentrifuge methods. It is shown that DNA chains bind only those nanoparticles whose surface has positive charge; DNA molecules will not form a complex with negatively charged nanoparticles. DNA complexes with spherical chitosan-coated PLGA particles by winding around them. The optimal DNA: nanoparticle ratio was found to be 1:7 by weight. This enables one nanoparticle to transfer about 3.5 × 105 base pairs of DNA to the cell cytoplasm.
Keywords: DNA delivery systems, poly(lactic-co-glycolic acid


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