þÿ<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <HTML> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> <title>JBPC Vol. 7, 2, 2007 ABSTRACT </title> </head> <body link="#0000FF"><center><h1><font color="#006600">The Journal of Biological Physics and Chemistry</font></h1></center> <p></p><p></p> <b><center>2007<p>Volume 7, Number 2, p.p. 51 57</center></b> <br> <div> <p><b><font size=+2> Chlorpromazine-membrane interactions as revealed by p<I>K<SUB>a</I></SUB> and fluorescent probes </font></b></p> <p> <b> V.M. Ioffe,* G.P. Gorbenko, O.K. Zakharenko and A.V. Yudintsev </b> <br> <br> <i> Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., 61077 Kharkov, Ukraine </i></p> <P align=justify> The effect of the cationic drug chlorpromazine (CPZ) on the structural state and physico-chemical properties of model lipid membranes composed of the zwitterionic phospholipid phosphatidylcholine (PC) and the anionic phospholipid cardiolipin (CL) at molar ratios 19:1, 9:1 and 4:1 has been investigated using the p<I>K<SUB>a</I></SUB> probe Neutral Red (NR) and the fluorescent membrane probes pyrene and 1,6-diphenyl-1,3,5-hexatriene (DPH). CPZ incorporation into the PC:CL lipid bilayers was followed by an increase of the NR partition coefficients. This effect was interpreted in terms of drug-induced enhancement of membrane hydration and alterations in headgroup molecular packing. Analysis of pyrene excimerization and DPH anisotropy data provided further evidence for the ability of CPZ to promote bilayer condensation. </p> <b>Keywords: </b> chlorpromazine, DPH, lipid bilayer, p<I>K<SUB>a</I></SUB> probes, pyrene </p> <br> </div> <p></p> <center><p><i><font size=-1><a href="jbpc20707.html">back to contents</a></font></i></p></center> </body> </html>