Volume 3, Number 2, pp. 45-48
M. Kurashvili, M. Pruidze, E. Kiskeidze, T. Varazashvili, T. Ananiashvili, G. Khatisashvili and M. Gordeziani
Durmishidze Institute of Biochemistry and Biotechnology, Georgian Academy of Sciences, David Agmasheneblis Kheivani 10 km, 350059 Tbilisi, Georgia
Influence of different factors on nitrobenzene oxidation
in the plant cell
Nitrobenzene oxidation has been studied in soybean (Glycine max) and maize
(Zea mays) root subcellular fractions, where the NADPH-dependent monoöxygenase
mechanism is of great importance. The influence of different factors on
nitrobenzene oxidation has been investigated. It was demonstrated that as
the plants age the monoöxygenase mechanism of nitrobenzene oxidation
was changed with peroxidase. The peroxidase and phenol oxidase systems were
activated due to the action of light against a background of monoöxygenase
reduction. In plants infected with Fusarium, the phenol oxidase system was
inhibited and the activities of the two other systems were sharply increased.
Low-temperature stress significantly blocked activities of all three oxidative
systems. N,N-dimethylaniline enhanced NADPH-dependent nitrobenzene hydroxylation
and 3,4-benzpyrene (benzopyrene) activated H2O2-dependent
nitrobenzene oxidation. Nitrobenzene as an inducer enhanced nitrobenzene
oxidation via both the monoöxygenase and peroxidase mechanisms.
Keywords: cytochrome P450-containing monooxygenase; peroxidase; phenol
oxidase; xenobiotic